Investigation and dot-ELISA Pathogen Identification of Virus Diseases in Pepper and Cherry Tomato in Hainan

Objective The study was conducted to investigate and identify the occurrence of virus diseases and virus types of peppers and cherry tomatoes in Hainan Province, and to determine the degree of damage and source of virus.

Method From 2018 to 2019, the symptoms, incidence rates and disease indexes of the viruses were analyzed for peppers and cherry tomatoes in 10 cities and counties of Hainan Province by 5 point sampling method. A total of 593 virus samples were collected from the main production areas of pepper and cherry tomato, to detect cucumber mosaic virus(CMV), tobacco mosaic virus (TMV), turnip mosaic virus (TuMV), broad bean wilt virus (BBWV) and cucumber green mottle mosaic virus (CGMMV) by using the dot enzyme-linked immunosorbent assay (dot-ELISA).

Result The main symptoms of capsicum virus disease were characterized by mottled mosaic leaves and dwarf twigs, the incidence rate was 0-75%, and the disease index was 0-25.67. The main symptoms of cherry tomatoes were shrinkage and curling with mottled floral leaves. The incidence rate was 7%-98%, and the disease index was 1.67-37.33. The total detection rate of pepper virus was 37.5%, the compound infection rate was 2.08%, while the total detection rate of cherry tomato virus was 20.33%, and the compound infection rate was 0.98%.

Conclusion Virus diseases are common in peppers and cherry tomatoes. The study results confirmed the disease status of pepper and cherry tomato in the field. The results of dot-ELISA showed that CMV and TMV were still the dominant virus sources among the five viruses detected, and there were four types of compound infection.