Screening and Identification of Salt-tolerant Bacteria Strains in Rhizosphere Soils from Reclamation Area in Shenzhen

【Objective】Salt-tolerant bacteria are widely distributed in saline soils，and the study of their distribution characteristics can provide useful reference for further improving saline soil and reducing plant salt damage. 【Methods】 10 soil samples were collected from shrub rhizospheres of reclamation areas in Baguang and Bao' an, Shenzhen. Salt-tolerant strains were isolated by NaCl media of different concentrations, and identified by electron morphologic observation and genomic DNA amplification sequencing. 【Results】A total of 66 strains were isolated from shrub rhizosphere soil, of which 14 strains had strong salt-tolerance and could grow at 10%-14% NaCl concentration. The results of electron microscopy and 16S rDNA sequence analysis showed that salt-tolerant bacteria were abundant and different in morphology in reclamation areas of Baguang and Bao'an, Shenzhen. Among the 14 bacteria, 13 strains were from Bacillus and 1 strain was from Lysobacter. In addition, the analysis of carbon utilization capacity of 3 salt-tolerant bacteria showed that the 3 bacteria had different carbon utilization capacity, and NY12 had the most available carbon sources. 【Conclusion】14 strains with strong salt-tolerance were isolated from the shrub rhizospheres for the first time, and their morphology and carbon utilizationcapacity were significantly different.