Determination and Comparison of Blood Physiological and Biochemical Indexes Between SPF BALB/c Mice and C57BL/6Mice in Different Physiological Stages and Genders

【Objective】The study was conducted to measure and compare the blood routine and blood biochemical values of 6 weeks old and 9 weeks old BALB/c mice and C57BL/6 mice.【Methods】Blood routine and biochemical values were determined by automatic blood cell analyzer and automatic biochemical analyzer.【Results】(1) There were extremely significant differences in 2 blood physiological indicators(NE% and LY%) of heterosexual BALB/c mice at the same physiological stage , and there were significant differences in 8 indicators (LY%, HGB and etc.) in homosexual mice at different physiological stages. There were significant differences in 7 blood physiological indicators (RBC and WBC and etc.) of heterosexual C57BL/6 mice at the same physiological stage, and there were significant differences in 6 indicators (WBC and NE and etc.) of homosexual mice at different physiological stages. (2) There was no significant difference in the biochemical indexes between the heterosexual 6 weeks old mice , with the changes of physiological stages, and there were extremely significant differences in 8 blood biochemical indicators (AST, ALP and etc.). There was no significantdifference in the biochemical indexes of heterosexual C57BL/6 mice at the same physiological stage , but there were significant differences in 7 blood biochemical indicators (ALP, GLU and etc.) . (3) With the changes of physiological stages, there was an increase in the difference in part of blood physiological and biochemical indicators of BALB/c mice and C57BL/6 mice. 【Conclusions】The physiological and biochemical indexes of mice blood are significantly affected by the physiological stage and gender of mice.