Study on Key Anti-freeze Genes and Pathways ofLanzhou Lily (Lilium davidii var. unicolor)by Transcriptome Sequencing

【Objective】 Lily (Lilium) is one of the first medical and edible plants published by the Ministry of Health, and is also a famous ornamental plant. As low temperature freezing damage seriously affecting the yield and distribution of plants, the study was to explore the molecular mechanism of the response of Lanzhou lily to subzero temperature stress. 【Method】 By using Illumina HiSeq transcriptome sequencing platform, sequencing was conducted to Lanzhou lily (L. davidii var. unicolor) exposed to two different temperature conditions, including, normal temperature treatment at 20℃ (CK) and cold treatment at -4℃ (D), and the sequencing data were analyzed.【Result】After being treated for 24 h, a total of 24 465 differentially expressed genes (DEGs) were identified, which included 4 143 significantly upregulated genes and 4 415 significantly downregulated genes, accounting for 10.27% of the total number of DEGs.【Conclusion】After enrichment analysis, DEGs related to protein kinase, protein phosphatase, carbon metabolism and ABA could be selected as main research objects for further studying the subzero cold response mechanism of Lanzhou lily. qRT-PCR analysis showed that the expression trend of 10 randomly selected DEGs coincided with the results of high-throughput sequencing. In addition, indol-3-glycerophosphate synthase, protein phosphatase, glycokinase, calcium-binding protein, and chlorophyll a/b binding protein were also selected as candidate genes that related to the anti-freeze responses of Lanzhou lily, Which lay a foundation for revealing the underlying mechanism of anti-freeze molecular of Lanzhou lily.