Isolation and Identification of Endophytic Fungi from Aleurites montana and Determination of Its Phosphate Solubilizing Capacity

【Objective】 The endophytic fungi from the Aleurites montana was isolated and identified, and the endophytic fungi with high phosphate-dissolving activity were initially screened.【Method】 Endophytic fungi were isolated and purified from roots, stems and leaves of Aleurites montana collected from the Waidun breeding ground, Huqiao work area and the Shufang forest farm in Jianyang District of Nanping City, Fujian Province. Then the dominant strains were screened according to the frequency of occurrence of colonies in roots, stems and leaves and the growth rate of their diameters, and the qualitative determination of the ability of solubilizing the dominant bacteria was further carried out. 【Result】 A total of 155 strains were isolated, among which, 60 strains of endophytic fungi were isolated from stems (38.71%), 50 strains from leaves (32.26%), and 45 strains from roots (29.03%). The number of endophytic fungi collected from the Huqiao sample area was the largest, while that from the Waidun sample area was the least. 25 dominant strains were identified as Mucor sp., Penicillium sp., Pestalotiopsis sp., Bionectria sp., Trichoderma sp., Thermomyces sp., Alternaria sp., Coniothyrium sp. and Fusarium sp. by morphological characteristics and 18 SrDNA molecules identification. Experiments on the effect of phosphorus solubilization of dominant strains showed that the strains with strong phosphorus solubilizing activity belonged to Penicillium sp. and Thermomyces sp. 【Conclusion】 The number of endophytic fungi in A. montana varied in different locations and among different organs. The strains with strong phosphorus solubilizing activity belonged to Penicillium sp. and Thermomyces sp..