Establishment of in vitro Regeneration System of Lilium Sorbonne and Siberia

【Objective】The suitable induction medium, proliferation medium, rooting medium and the optimal transformation receptor system were determined for the in vitro regeneration systems of Lilium Sorbonne and Siberia.【Method】 The bulbs of Sorbonne and Siberia were used as explants to compare their growth status in the medium with different hormone ratios. And the optimal conditions of callus and adventitious bud induction, subculture proliferation, induction/rooting medium and kanamycin sensitivity were obtained.【Result】The best induction medium for Sorbonne was MS+2,4-D 1.5- 2.0 mg/L, while the best induction medium for Siberia was MS+2,4-D 1.0-1.5 mg/L. The optimal subculture proliferation medium for the two Lilium species was MS+6-BA 0.5 mg/L+NAA 0.2 mg/L. The optimal rooting media for the two Lilium species were 1/2MS+NAA 0.2 mg/L and 1/2MS+IBA 0.2 mg/L, respectively. When screening transformed plants, kanamycin at a mass concentration of 150 mg/L was sufficient to inhibit the growth of non-transformed cells or bacteria.【Conclusion】 The theoretical basis was provided for the industrialized production and genetic transformation and regeneration system of Lilium oriental varieties.