Study on Rapid Propagation Technology for Caladium bicolor Tissue

【Objective】The research aimed to study the optimal hormone concentration for the rapid propagation of caladium bicolor tissue, and to provide reference and help for its factory production.【Method】Young leaves developing from C. hortulanum ‘Wightii’ and C. hortulanum ‘Postman Joyner’ were used as the explant to make an optimization experiment of callus induction and callus proliferation differentiation system, by taking tissue culture and propagation in different formulations of 6-BA and NAA on MS basal medium. 【Result】Different combinations of plant growth regulators showed different effects on callus induction, differentiation and proliferation within a certain mass concentration. All the C. bicolors could induce callus and regenerated plants in these different formulations. After transplanting to the matrix mixed with peat soil, vermiculite and perlite, the survival rate of seedling was more than 90%. 【Conclusion】The results showed that MS+6-BA 4.0 mg/L+NAA 0.5 mg/L was the best culture medium for callus induction of C. bicolor with short start-up time and high cure rate, while MS+6-BA 2.0 mg/L+NAA 0.2 mg/L was most effective for differentiation and proliferation with the highest callus differentiation rate and the best growth of test-tube seedlings.