Analysis on Microbial Diversity in Rhizosphere Soil of Davidia involucrate by High-throughput Sequencing

【Objective】The rhizosphere soil of Davidia involucrate in different regions was tested in order to understand the species and diversity of the rhizosphere microorganisms, and provide basic data and theoretical basis for the introduction and breeding of D. involucrate. 【Method】High-throughput sequencing was applied to study the microbial species and diversity of rhizosphere soil of D. involucrate in three regions of Zhaotong, and the species composition and abundance, Alpha diversity, Beta diversity and microbial community structure were analyzed. 【Result】The results showed that Alphaproteobacteria was the most abundant in the bacterial community (>17.02%), Fungal community was obviously different in three regions. Pezizomycetes was the most abundant fungi class (17.01%) in Yongshansanjiangkou, Archaeorhizomycetes was the most abundant fungi class (29.79%) in Yiliangxiaocaoba, Sordariomycetes was the most abundant fungi class(20.77%) in Zhenxiongtangfang. Among the three regions, the microbial community structures in Sanjiangkou, Yongshan and Tangfang, Zhenxiong were highly similar. The rhizosphere soil in Sanjiangkou, Yongshan reflected the richest bacterial diversity, while that in Tangfang, Zhenxiong mirrored the richest fungal diversity. 【Conclusion】 The microbial community in rhizosphere soil of D. involucrate in the three regions had high biodiversity, but there were differences in the distribution of species composition.