Study on bud induction and regeneration from leaves of Nongda No.5 in Chinese dwarf cherry

In this study, leaves of Chinese dwarf cherry variety Nongda No.5 were collected from greenhouse cultivated seedlings and tissue cultured seedlings respectively. Bud induction and plant regeneration were studied. Theoptimum medium for adventitious buds induction of leaves from greenhouse was 1/2MS+IAA 0.1 mg/L+NAA 0.05 mg/L+6-BA 0.8 mg/L, in which all the nutrients were halved.The best medium for adventitious buds induction of leaves from tissue cultured seedlings was1/2MS+IAA 0.05 mg/L+NAA 0.1 mg/L+6-BA 0.8 mg/L, in which macro-elements were halved. Moreover, auxiliary factor studies showed that the highest bud induction rate was achieved with the combination of adding 0.2 mg/L AgNO3 into the medium, full light exposure, turning leaves upside down and using leaves with petioles.