Relationship between the PRV release of porcine pseudorabies and the level of gE，gB antibody

Different samples including serum，milk， nasal swabs and so on，in different stages were collected from PRV-postitive and negative farm in present study，the level of gE and gB antibody were detected by ELISA， The result showed that the total positive rate of gE was 56.7%，only lactating sows and 1week piglets were negative， the level of antibody of 6 to 24-week pig increased gradually from 52.9% to 100%，while the effection rate was up to 90%-100% in sows including（perinatal and backup period sow） .The total level of gB antibody was 88.8%， the positive rate in sow and boar were up to 90%-100%，the positive rate of 1 week-old piglets was 100% and then gradually reduced to 40%，after that the positive rate increased quickly after 10 week. The negative farm showed that the gE was negative，and the positive rate of gB was 38.9%，especially for boar sow，their rate was up to 100%， the gB antibody turned to be negative from 2 week to 12 week，the gB positive rate of 14 week-old to 20 week-old fishing pigs was 20%-75%. RT-PCR was performed to detect PRV nucleotide，the result of positive farm showed a widely effection in the deferent herd，except semen，especially the PRV-positive rate in cord blood was highest， up to 80%，colostrum，33.3%，the positive rate of swabs were low，range of 0.55 to 6.21%. these results account for the various ways of shedding and release of PRV， vertical transmission is predominate method of transmission in our study，compared to negative，the PRV release not only was relationship with gE antibody，but also disturbed maternal antibodies and immune antibodies，the gE and gB antibodies were significantly abnormally increased when maternal and immune antibodies were fading away.