Cloning and sequence analysis of Actin gene fragment from Talinum paniculatum Gaertn

In order to provide reference gene for studying the expression pattern of functional genes in Talinum paniculatum，the cDNA fragment of Actin from Talinum paniculatum；was amplified by RT-PCR，by adopting degenerated primers designed according to the conserved region of the Actin genes which were submitted to NCBI database. The cDNA fragment was inserted into the pGEM-T vector and sequenced. The cDNA sequence was analyzed by online bioinformatics software. Our result showed that this actin cDNA includes 598 base pairs，encoding a partial actin with 198 amino acids. Sequence alignments showed that it shares over 85% nucleotide sequence identity and 86% amino acid sequence identity with other plant actins. This study indicated that a new actin cDNA fragment was isolated from Talinum paniculatum. This cDNA was named TpActin1 and submitted to GenBank （accession number: MH33309）.