Optimization of culture medium for plantlets of Cunninghamia lanceolata（ Lamb.） Hook. in tissue culture proliferation

Using Cunninghamia lanceolata（ Lamb.） Hook as material，through L9（34） orthogonal test design，the effects of MS medium，TDZ concentration and banana juice concentration on the proliferation coefficient，chlorophyll content and soluble sugar content of plantlets were studied. The results showed that：The effects on the multiplication coefficient of the plantlets in tissue culture were as follows：basic medium > banana juice concentration > TDZ concentration. The effect on the content of chlorophyll and soluble sugar was as follows：basic medium > TDZ concentration > banana juice concentration. It is found by comprehensive evaluation of coordinates that，3/4MS + banana juice 100 g/L was the best combination for the multiplication and culture of the plantlets in tissue culture. Under this condition，the proliferationg coefficient was 2.45，the chlorophyll content was 2.45 mg/g， the soluble sugar content was 33.47 mg/g.