Identification of an endophytic bacteria from Pennisetum sp. and the preliminary analysis of its growth promoting properties

Using Juncao as materials，endophytes were gained by tissue isolation and streak plate method. The ability of indole acetic acid（ IAA） production and siderophore production were tested by the Salkowski colorimetric method and CAS assay，separately. The ability of phosphate solubilization and nitrogen fixation were also detected by using Mo-Sb-Ascrobiology acid colorimetry and Ashby agar plates，separately. The strain was identified according to its physio-biochemical characteristics and the sequence analysis of 16S rRNA gene. The results showed that a total number of 86 strains of endophytes were isolated from Juncao，YA-6 strain had the highest ability to produce IAA （11.73 mg/L）. This strain also had characteristics of phosphate solubilization（ 7.103 mg/L），nitrogen fixation and siderophores production. Endophytic bacteria YA-6 was rod-shaped and gram-negative. The sequence analysis of 16S rDNA gene showed that strain YA-6 belonged to Pseudomonas hunanensis with 99.36% identity. YA-6 strain had many growth promoting properties，which was expected to be new microbial fertilizer.