Induction culture of callus and bud in Pachysandra terminalis

In order to rapidly propagate elite varieties of Pachysandra terminalis and screen out optimal culture conditions for callus and bud induction ，we studied the effects of different culture conditions on induction of callus and bud in P. terminalis including different sampling time，disinfecting methods of explants，hormone concentration andlight treatment，taking annual tender stem with lateral buds from P. terminalis as explants. The results showed that the best sampling season was May which provided acallus induction and bud induction rate of explants of 44% . Better disinfection effect could be achieved by disinfecting with 0.1% HgCl2 for 6 min，of which the pollution rate and the death rate was 11% and 22% respectively. The optimal concentration of hormone for inducing medium of callus and buds was 0.5 mg/L NAA+2.0 mg/L 6-BA，with an induction rate of 47%. Light culture is better than dark culture， with an induction rate of 60%.