Diversity analysis of FTO gene and their association with meat quality traits in Huai Pig

PCR-RFLPs with EcoR Ⅰ，Xap Ⅰ and Bsh1236 I restriction enzymes were used to analyze genetic diversities of three SNPs at the 5′-upstream region（g.-167 T>G），the first intron（c.46-139A>T） and the third exon（c.594C>G） of the FTO gene，and potential associations between genotypes and three meat quality traits including intramuscular fat（IMF），backfat thickness（BFT） and loineye area（LEA） were investigated. The results showed that three SNPs presented certain degrees of diversities with the PIC value being 0.276，0.343 and 0.235，respectively；and the frequencies of observed genotypes conformed to Hardy Weinberg equilibrium （P>0.05）；for g.-167 T>G SNP，G allele was dominant with the frequency being 0.790，whereas CC genotype was statistically raised IMF content；for c.46-139A>T SNP，T allele was dominant with the frequency being 0.674，and TT genotype was statistically decreased BFT comparing toAA genotype；for c.594C>G SNP，G allele was dominant with the frequency being 0.837，and the SNP were not statistically associated with tested traits. In summary，two SNPs of g.-167 T>G and c.46-139A>T could be potential molecular genetic markers for the meat quality traits in the population of Huai pigs.