Study on proliferation conditions and resistance of Bacillus coagulans

The study was conducted to investigate the proliferation and resisitance of Baclillus coagalans. The results showed that：the optimum medium formula included sodium chloride 2 g/L，peptone 5 g/L，yeast powder 25 g/L，maltose 6 g/L，magnesium sulfate 1.2 g/L，manganese sulfate 0.3 g/L，sodium phosphate dibasic∶ sodium dihydrogen phosphate 3.0 g/L∶5 g/L；the optimum culture conditions were as folbous: the initial pH value of 7.4，180 r/min，at 37℃ for 20 h speed training，the total number of colonies can reach 3.8×1011 CFU/mL. B. coagulans resistance values：D80℃ = 82.24 min，R2 80℃ = 0.9943，D90℃ = 47.92 min，R2 90℃ = 0.9955，D100℃ = 16.92min，R2 100℃ = 0.9944. The thermal death curve equation was y = -3.266x + 342.96. In the environment of pH value of 2～6 and 0.2%～1.4% pig bile salt solution，the survival rate of B. coagulans was 100%. The aim of this experiment is to provide basis for the application of this strain in feed fermentation industry.