Identification and bioinformatics analysis of 14-3-3ζ gene from Monochamus alternatus

In order to elucidate the characteristics of Monochamus alternatus，the 14-3-3ζ gene from M. alternatus called Ma14-3-3ζ was cloned by using cDNA library construction，GenBank（KT970073.1）. The cloned cDNA of Ma14-3-3ζ is 902 bp in length，which contains a 747 bp open reading frame（ ORF） encoding 248 amino acids with a calculated molecular mass of 28.16 KD and an isoelectric point of 4.73. The results also indicate that Ma14-3-3ζ show the highest homology with Tenebrio molitor（ 98%），and has 95%-90% amino acid sequence homology with the 14-3-3ζ from 12 species of insects such as Nilaparvata lugens，M. alternatus and T. molitor on the same branch in the phylogenetic tree based on insect 14-3-3ζ analysis.