Advances in clusterd buds tissue culture of Phalaenopsis

The Phalaenopsis market has broad prospects，the rapid breeding of seedlings by tissue culture is the only method for the mass production of Phalaenopsis. The clusterd buds tissue culture of Phalaenopsis was reviewed in order to provide a basis for the scale production. As the explant in section 2 or 3 of the pedicel，0.1% chlorinated mercury disinfected 8-15 min was better than sodium hypochlorite. The basic medium of 1/2MS or hyponex No.1， and 6-BA or 6-BA and NAA could be used to induce clumps. 6-BA or TDZ and the appropriate concentration of NAA had a significant effect on the proliferation of clusterd buds，and the basic culture medium was either MS or hyponex No.1. The low concentration of NAA or IBA，and the organic matter such as coconut juice or banana juice， potato juice and so on had a good effect on the rooting. The activated carbon could inhibit browning and the influence of browning was first，and the influence of transition cycle on browning was secondary.