Primary histocyte culture and chromosome karyotype analysis of Pomacea canaliculata

Pomacea canaliculata’s muscle，mantle，kidney and brain tissues were aseptic separated by trypsin digestion and grinding method. Cells were primary cultured with DMEM，F12 and modified 1640 medium. The growing status was observed under light microscope. The cell viability was determined by MTT method. After a certain time，chromosome preparation and chromosome karyotype analysis were conducted. Results showed that there was significant difference in histocytes viability among different culture medium. The better viability of mantle and muscle histocytes was cultured with DMEM medium containing hydrolyzed milk protein. The better viability of mantle and brain histocytes was cultured with M199 medium. In karyotype analysis，results showed that P. canaliculata was diploid（ 2n = 28，NF = 56）. The nuclear type was 2B and the nuclear asymmetry coefficient was 66.4%