Study on ultrasonic assistant water extraction processing of Bergamot oligosaccharides

Using preserved Bergamot as raw material，the extraction technology of ultrasonic assisted-water was studied. To optimize the extraction process of bergamot oligosaccharides technology，extraction time，ultrasonic time， extraction temperature，ratio of material to watek chosen as factors which affected the extraction rate by orthogonal test. Considering the results of single factor experiment，orthogonal experiment results and energy conservation，the optimum extraction technology was as follows: extraction time 2 h，ultrasonic time 20 min，extraction temperature 80℃，and ratio of material to water 1∶8. The yield of bergamot oligosaccharide can reach 5.99（±0.15）% under these conditions. The capabilities of bergamot oligosaccharide which reduced blood lipid function was estimated as index by bile salt-binding experiment. The results suggested that bergamot oligosaccharide presented stronger binding ability to sodium cholate（ SC），sodium taurocholate（ STC），which accounted for 20.30（±0.39） μmol/mg，19.61（±0.71）μmol/mg，respectively，while it presented weaker binding ability to glycocholate （SGC），which accounted for 14.38（±2.23）μmol/mg. Therefore，bergamot oligosaccharide have function of reducing blood lipid.