Improvement and analysis of cactus total RNA extraction method

High-quality total RNAs of cactus stem were obtained by using Na2SO3 with each of TRIzol reagent， RNA plant plus reagent，BioTeke total RNA extraction reagent，EASY spin Plus plant RNA extraction reagent （Aidlab） or OMEGA plant RNA reagent. The cDNA RT-PCR and specific detection results showed that matK gene fragments were amplified by five total RNA extraction methods. The cDNAs that reverse transcribed from the TRIzol reagent，RNA Plant Plus Reagent，and EASY spin Plus Plant RNA Extraction reagent（ Aidlab） were relatively high，as the sensitivity PCR of cDNA highest dilution to 1∶100. While the cDNAs that reverse transcribed from BioTeke total RNA extraction reagent and OMEGA plant RNA reagent were relatively low，as the sensitivity RTPCR indicated the cDNA highest dilution to 1∶10. In this study，Na2SO3 combined with different plant total RNA extraction kits to prepare high quality of RNA is the key of cactus stem cDNA library preparation and molecular biology.