Advances in suspension-cultured methods of soybean cell and accumulation on soybean isoflavones and glyceollins from cell exposed to elicitor

Suspension-cultured cell technique which developed soybean cell and aggregates in liquid medium， has been extensively applied in numerous fields. The paper summarized the methods for suspension-cultured soybean cell employing different explants，including cotyledon，cotyledon node，hypocotyl，immature embryo and stem， inducible effect of exogenous elicitors and precursor treatment on accumulation of isoflavones and influence of elicitors exposure to soybean cell on synthesis and accumulation of glyceollins. Soybean isoflavones and glyceollins are two types of bioactive secondary metabolites produced from soybean. On the basis，the present problems were illustrated and prospective development directions were also recommended for the propose of providing ideas concerning higher level of isoflavones and glyceollins accumulation produced by cell in response to other optional elicitors.