Screening of lignin degradation strain MZ-9 and optimization of enzyme production conditions in compost

This experiment use the potassium ferricyanide color，guaiacol color and aniline blue（ Azure - B） fading circle method，and liquid fermentation experiment，measured enzyme activitives of Laccase（ Lac） and Manganese Peroxidase（ MnP） were as high as 9.53 U/mL，9.53 U/mL，lignin degradation rate was 20.32%，from the compost. An active strain MZ-9 is screened as a strong lignin degradation strain. Physiological and biochemical tests and 16S rDNA sequence analysis showed that strain MZ-9 belonged to Bacillus and had 99.2% similar to Bacillus licheniformis. Enzyme production showed that when the pH value was 8.0，the culture temperature was 37℃， the inoculation amount was 8% and the culture time was 6 d，the Lac and MnP activities of strain MZ-9 were the highest，and the degradation rate of lignin was 20.51%. Therefore，strain MZ-9 is an excellent lignin degradation strain，has a very good development and utilization value in the lignin degradation，and can provide a reliable strain resource support for the subsequent industrial production.