Cloning and expression analysis of NoZIP1 gene in Nasturtium officinale R. Br.

To contribute to the further functional determination of the gene involving growth and development of Nasturtium officinale R. Br.，ZIP1 gene was cloned according to homologous gene conserved region by RACE method. Its sequences and expression characters were examined by bioinformatics and real-time PCR， respectively. The full-length cDNA was 1 239 bp and it encoded a polypeptide of 356 amino acids with an entire ORF. It had high similarity to ZIP proteins of other plants. By real-time PCR approach，it was found that the expression of NoZIP1 was significantly induced both in roots and shoots by zinc-deficiency or iron-deficiency.