Vector construction， prokaryotic expression and resistance to heavy metals of OsMT-1-4c

In order to study the resistance of paddy rice to heavy metals， PGEX4T-3-OsMT-1-4c， the prokaryotic expression vector of metallothione for paddy rice was constructed， which was then transformed into strain Rossetta to induce the expression， about 33.643 kDa of GST-OsMT-1-4c fusion protein was obtained. The obtained precipitation was dissolved with 1×PBS and conducted ultrasonic fragmentation at low temperature， followed by the centrifugation and electrophoresis detection， and then was gained through the purification. Furthermore， when the recombinant vector and empty vector strains were induced to express under the stress of heavy metal Cd2+， it was found that the strain to express protein GST-OsMT-1-4c owned stronger resistance to heavy metals than that of protein GST.