Effects of different accelerated solvent extraction conditions on lipids extraction from Chlorella sorokiniana

Response surface analysis（ Box-Behnken） was carried out to investigate the effects of three factors on lipids extraction from Chlorella sorokiniana using accelerated solvent extraction technique. Factors presented here were extraction temperature，extraction time and chloroform-methanol volume-ratio. Based on experimental data，ANOVA and quadratic regression reswts showed that， model was significant（ P ＜ 0.0001），in which lack of fit was not significant and R2 was 0.9917，close to 1 and R2ad（j 0.9811）. ANOVA also indicated that the effects of extraction time and extraction temperature on lipids extraction were significant，while that of chloroform-methanol volume-ratio was not. Elliptic contour plots showed the presence of interaction between factors. The highest amount of extract of predicted and experimental values were 16.06% and 16.10%，corresponding condition in which extraction temperature was 132.37℃，extraction time was 12.79 min and chloroform-methanol volume-ratio was 2.26∶1.