Optimization of γ-aminobutyric acid extraction from mulberry leaf by response surface methodology

By seriatim-factorial experiment and Box-Behnken test，the extraction conditions of γ-aminobutyric acid（GABA） from mulberry leaf with water were optimized. The results showed that the extraction rate was 0.464% under the following modification conditions: the ratio of mulberry leaf powder to water was 1（g）∶30（mL） and treated at 50℃ for 2.13 h. The order of the factors influencing the result was extraction time﹥ratio of mulberry leaf powder to water﹥temperature.