Polymorphism and association analysis of NKX2-5 gene with shank length in three chickens

Gaojiao chicken，Aijiao chicken and Baiyi black chicken in Guizhou province were used as experimental subjects in this study. SNPs of NKX2-5 genes were detected in three chicken vareties by PCR-SSCP and DNA sequencing technologies. The 2 SNPs were detected in the 3 chicken groups，T563C，C675T sites of NKX2-5 gene were missense mutations，The relevance between T563C site of NKX2-5 gene and chicken shank length showed that，TT genotype was significantly higher than CC genotype among Gaojiao chicken groups（P < 0.05），TT genotype was significantly higher than CT genetype in Baiyi black chicken groups（P < 0.05），uhile no significant level aruong 3 genotypes in Aijiao chicken. The C675T site of NKX2-5 had only CC and CT genotypes，and there was no significant level in different genotypes of 3 vareties.