Cloning of GmMYB138A from Glycine max. and its expression analysis under cold stress

According to the soybean genomic database，a transcription factor gene，GmMYB138A，was cloned from Glycine max. cv Jilin32. Bioinformatic analysis indicated that the protein encoded by this gene belonged to 1RMYB group，and was localized in nucleus. Sequence alignment and phylogenetic analysis showed that GmMYB138A was clustered into the 1R-MYB group，evolutionarily closest to MYB138 from soybean，and the identity was up to 96%. Furthermore，q PCR analysis was conducted to examine its expression pattern in response to cold stress. As a result，the transcriptional level was gradually promoted with the cold treatment duration，and the peak occurred at 24h after cold stress，suggesting that GmMYB138A might be involved in the signaling transduction during cold stress.