Cloning and expression analysis of cell wall degrading enzyme genes from banana soft rot pathogen

Cell wall degrading enzymes had important relationship with the symptoms of plant diseases. Two pairs of specific primers were designed for amplification and cloning gene sequences of Banana soft rot pathogen according to reported cell wall degrading enzyme genes. A complete reading frame sequence of gene pelD and pelE were obtained by cloning technology. The expression vector pET28b（ +） was used to investigate the pelD and pelE gene expression.According to the results of SDS-PAGE，under the inducing of IPTG，transformants of recombinant expression vectors expressed much protein. After the purified protein was inoculated into banana false stem and leaves in vitro，similar rot symptoms were presented compared to Banana bacterial soft rot pathogen，the purified protein from vector pET28b（ +）-pelE after expressing 12 h appeared serious rot，while the purified protein from vector pET28b（ +） –pelD did not cause rot in plant.