Primary research on anther culture of photo-thermosensitive genic male sterile rice “Y58S” and selection of descendant of anther culture

The combination of anther culture techniques and traditional sterile lines breeding can effectively speed up the improvement of photo-thermosensitive genic male sterile（PTGMS） lines. To screen for the optimal culture medium for the two-line male sterile line， Y58S，we studied the effects of 10 different culture mediums on the F2 generation of different cross combination between Y58S and selected male parents. The results showed that medium E（NMB+2 mg/L 2，4-D +2 mg/L NAA+1 mg/L KT+0.5 g/L Pro+0.5 g/L Gln+0.5 g/L CH+25 g/L sugar+25 g/L Maltose+10 g/L Agar）was more effective for anther culture of PTGMS lines. And introducing japonica rice by cross breeding improved anther culture ability of PTGMS lines，indicating that genetype played an important role in rice anther culture. The examination on traits in field of descendants displayed that most of the plants were diploid， some were haploid and tetraploid， and no non-integer ploidy，and a new PTGMS lines was screened.