Objective The natural propagation mode for Murraya paniculata is sowing, which can not meet the needs of extensive production of plantlets. The establishment of efficient in vitro regeneration system lays a foundation for industrialized breeding of tissue culture seedlings for M. paniculata.

Method Mature seeds of M. paniculata were used as explants to study the effects of different concentrations and ratios of hormone and different organic matters on seed germination, proliferation, strong seedling culture and rooting culture of M. paniculata. Then, sterile tissue culture seedlings were obtained for transplanting.

Result After the pretreatment with 75% alcohol and 0.1% HgCl₂, the most suitable medium for seeds germination is MS+6-BA 0.5 mg/L, and the germination rate is 62.50%; the most appropriate basic medium for subculture proliferation of cluster buds is the MS medium; the most suitable medium for multiplication culture is MS + 6-BA 0.50 mg/L + NAA 0.15 mg/L, and the multiplication coefficient is up to 2.37; the suitable medium for strong seedling culture is MS + potato juice 30 g/L, and the plant height increase to 2.0 cm in 40 days; the optimal medium for rooting culture is 1/2MS + IBA 0.6 mg/L + potato juice 30 g/L+ banana juice 30 g/L + AC 0.1 mg/L, and the rooting rate is 55.6%.The survival rate of the rooting seedlings is 85% after domesticated and transplanted into the mixed matrix(peat soil: loess: perlite: fertilizer of calcium, magnesium and phosphate, 3∶2∶1∶0.2).

Conclusion Different hormones and concentrations have significant effects on seed germination and cluster bud proliferation. Adding potato juice and banana juice are beneficial to the growth of plantlets and rooting.