Objective The contents of important active ingredients such as chlorogenic acid, rutin, and solanesol in different tobacco varieties for propagating were detected by HPLC method with a view to providing references for the effective development and utilization of tobacco germplasm resources.

Method The contents of chlorogenic acid, rutin, and solanesol in leaves of 17 tobacco germplasm resources for propagating at mature stage were determined by HPLC. The chromatographic conditions were as follows: chromatograph was Agilent 1260, chromatographic column was VP-ODS, flow rate was 1.0 mL/min, the column temperature was 35℃ and the detector was 1260 VWD.

Result The content of chlorogenic acid was the highest among the six isomers, and was detected in all 17 germplasm resources, and that of S16 Dabinliuyeyan sun-cured tobacco was the highest, followed by cryptochlorogenic acid and neochlorogenic acid. Compared with the first three isomers, the content of isochlorogenic acid A, isochlorogenic acid B and isochlorogenic acid C was generally lower. The content of rutin in NC347 flue-cured tobacco was the highest(46.456 g/kg), followed by S19 Dajianye (7.782 g/kg)and S17 Daduqinggeng(6.250 g/kg)sun-cured tobaccos, and that of Yunyan 87 flue-cured tobacco was the lowest(1.206 g/kg). The content of solanesol in NC347 flue-cured tobacco was the highest(44.913 g/kg), followed by K326 flue-cured tobacco(11.140 g/kg)and S17 Daduqinggeng air-cured tobacco(9.844 g/kg), and that of Huangkeng No.7-1 flue-cured tobacco was the lowest(2.793 g/kg).

Conclusion Among the 17 tobacco germplasm resources, the content of rutin and solanol was higher than that of chlorogenic acid, and the highest level was found in NC347 flue-cured tobacco. The content of chlorogenic acid was the highest among the six isomers, among which the S16 Dabinliuyeyan sun-cured tobacco had the highest content. The tobacco germplasm resources for propagating did not topping after the appearance of flower-buds, which may have a greater effect on chlorogenic acid.