Objective The cucumber germplasm resources collected by our group were investigated to provide references for the development and utilization of these resources.

Method Based on 197 cucumber germplasm resources collected by our research group, 10 agronomic traits were investigated and observed, and diversity, principal component analysis and cluster analysis were carried out.

Result The results of diversity analysis showed that the 197 cucumber germplasm resources had rich genetic diversity, and the average coefficient of variation was 22.59%, among which the coefficient of variation of the first female flower node was the largest(48.35%), and the coefficient of variation of cotyledon width was the smallest(10.03%). The results of principal component analysis showed that there were 3 principal components with eigenvalues greater than 1, among which the first principal component contained leaf and branch factors, the second principal component mainly contained hypocotyl and cotyledon seedling stage factors, and the third principalcomponent included the first female flower node and leaf color. Cluster analysis showed that the 197 cucumber germplasm resources could be divided into 4 major groups, among which group I could be used to select varieties with long hypocotyls, Group II used to select varieties with smaller leaves, Group III used to breed varieties with larger cotyledons, and Group IV used to breed varieties with smaller cotyledons and leaves.

Conclusion Cucumber germplasm resources preserved by our group have a rich diversity of agronomic traits, and there is a certain correlation between different agronomic traits. The germplasm resources of different groups can be used for corresponding cucumber breeding.