堆肥中木质素降解细菌MZ-9 的筛选及产酶条件优化
Screening of lignin degradation strain MZ-9 and optimization of enzyme production conditions in compost
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摘要: 在堆肥中依次采用铁氰化钾显色、愈创木酚显色、苯胺蓝（Azure-B）退色圈试验以及液体发酵验证试验测得漆酶（Lac）、锰过氧化物酶（MnP）的活性分别高达9.53 U/mL、21.56 U/mL，木质素降解率达20.32%，从而筛选到一株高活性木质素降解菌株MZ-9。生理生化试验及16S rDNA 序列分析表明，菌株MZ-9 属于芽孢杆菌属（Bacillus），与地衣芽孢杆菌（Bacillus licheniformis）相似性为99.2%。产酶条件试验表明，当pH 值8.0、培养温度37℃、接种量8%、培养时间6 d 时，菌株MZ-9 的Lac、MnP 活性最高，此时木质素降解率为20.51%。因此，菌株MZ-9 是一株优良的木质素降解菌株，在木质素降解方面具有很好的开发利用价值，可为后续的工业化生产提供可靠的菌种资源支撑。Abstract: This experiment use the potassium ferricyanide color，guaiacol color and aniline blue（ Azure - B） fading circle method，and liquid fermentation experiment，measured enzyme activitives of Laccase（ Lac） and Manganese Peroxidase（ MnP） were as high as 9.53 U/mL，9.53 U/mL，lignin degradation rate was 20.32%，from the compost. An active strain MZ-9 is screened as a strong lignin degradation strain. Physiological and biochemical tests and 16S rDNA sequence analysis showed that strain MZ-9 belonged to Bacillus and had 99.2% similar to Bacillus licheniformis. Enzyme production showed that when the pH value was 8.0，the culture temperature was 37℃， the inoculation amount was 8% and the culture time was 6 d，the Lac and MnP activities of strain MZ-9 were the highest，and the degradation rate of lignin was 20.51%. Therefore，strain MZ-9 is an excellent lignin degradation strain，has a very good development and utilization value in the lignin degradation，and can provide a reliable strain resource support for the subsequent industrial production.
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