Abstract: In order to solve the problem of growing difficulties of Anoectochilus roxburghii（ Wall.） Lindl in Guangdong Province，the tissue culture plantlets of Zhaoqing germplasm were used as materials，the integrated control techniques were studied，such as the pretreatment of seedings，selection of matrix and planting density， greenhouses daily maintenance，biological characteristics of diseases and insect pests. Especially in the diseases and pests control，the results showed that there was the best control efficacy，by the combination of 5000 times of 43% Tebuconazole E.C+3000 times of 73% propargite +5000 times of 10% imidaclporid，the control efficiency reached 83.3%. The growth index of A. roxburghii such as plant height，leaf quality，stem diameter，fresh dry weight were better than other treatments，the individual drying rate was 13.36%. This technology system of planting the endangered medicinal A. roxburghii in greenhouse，provided technical basis for the large-scale cultivation of A. rocburghii in Guangdong Province.