Abstract: Through the use of broccolini innovation resources, a group of unique excellent broccolini and male sterile lines, as well as inbred lines were bred. 4 hybrid strains were bred by the use of male sterile lines which had vigorous growth, high resistance, good quality, good commodity and high yield, including Beauty No. 1, Beauty No. 2, Beauty No. 3, Beauty No. 4. The results showed that Beauty No. 2 behaved the best comprehensive characters of high yield, good commodity and good taste. The yield of Beauty No. 2 was 1 879 kg per 667 m2 and 25% higher than the control Qianxiu, the sulforaphane content was as high as 48 mg/kg and 77.8%, 272.1% higher than the control of Qianxiu and Manuolv broccoli, respectively. The comprehensive character of Beauty No.1 ranked the second, the yield was the highest (1 951 kg per 667 m2) and 30% than the control Qianxiu.