Abstract: In the field， under different irrigation conditions， the secondary substances of Lycium barbarum accumulation were studied. The results showed that adequate water supply was conducive to the accumulation of total sugar and Vc， and drought treatment was conducive to the accumulation of betaine and carotenoids， but not conducive to the accumulation of Vc， flavonoids and sugar. Correlation analysis showed that there was positive correlation between polysaccharides content and betaine content， no significant negative correlation between betaine content and carotenoids content， significantly positive correlation between betain contente and total sugar content. There was very significantly negative correlation between moisture content and polysaccharide / Vc content， significantly negative correlation between moisture content and polysaccharide / flavone content， negative correlation between moisture content and polysaccharide/betaine content， negative correlation between moisture content and polysaccharide/carotenoid content. Resurts indicated， proper irrigation could not only guarantee the quality of L. barbarum L.， but also decreased the output reduction of L. barbarum L. In this experiment， the reasonable frequency of irrigation for L. barbarum L.， growing period was 2-3 times， the amount of water per 667 m 2 was 75m 3 .