不同基因型甘蔗耐低钾能力、 光合特性和抗氧化酶系统的差异
Differences in low-K tolerance, photosynthetic characteristics and antioxidant enzyme system between different sugarcane genotypes
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摘要: 以粤糖 60 号(YT60)和斑茅杂交后代 BC2-32 为材料, 采用营养液培养研究其耐低钾能力、 光合特性、 叶绿素荧光参数及抗氧化指标的差异。 结果表明, 与正常钾水平相比, 低钾胁迫下, BC2-32 的干物重下降了 59.39%, 而 YT60 的干物质量只下降了 30.61%, 下降幅度明显小于 BC2-32, 表现出较强的耐低钾能力; YT60 叶片叶绿素含量、 净光合速率(Pn)和最大光化学效率(Fv/Fm)的下降幅度均比 BC2-32 小, 而且超氧化物歧化酶(SOD)活性、 脯氨酸和丙二醛(MDA)含量的升高幅度也较小, 表明光合特性以及抗氧化酶系统相关指标可以作为伸长前期筛选耐低钾基因型甘蔗的指标。Abstract: A hydroponic experiment was conducted to investigate the differences of low-K tolerance ability, photosynthetic characteristics, chlorophyll fluorescence parameters and antioxidant indexes between Yuetang 60 (YT60)and BC2-32, a progeny of Erianthus arundinaceus. The results showed that there was decline with 59.39% in dry weight of BC2-32 under low K stress, while only 30.61% for YT60, indicating that YT60 was more tolerant to low K stress than BC2-32. Under low K stress, the decline of leaf chlorophyll content, net photosynthetic rate(Pn)and maximum photochemical efficiency(Fv/Fm)was less and the increase of SOD activity, proline and malondialdehyde (MDA)content was also less for YT60 compared to BC2-32, which demonstrated that the photosynthetic characteristics and related indexes of the antioxidant enzyme system could be used as the indicators for screening sugarcane genotype with low K tolerance.