Abstract: The potential of mesophilic anaerobic digestion for the treatment of navel orange wastes and swine manure was evaluated. Co－digestion process was conducted in batch digesters. Effects of total solid（TS），C/N， and temperature on co－digestion process were studied by orthogonal experiment. Results showed that high－level accumulation of volatile fatty acid（VFAs）and lack of pH－regulation might contribute to poor methane production. According to intuitive analysis，the most influent factor for biogas production was C/N，follow by TS，then temperature. The optimum condition was C/N of 35，TS of 15% and at 55℃.