水稻冷诱导蛋白激酶基因CIPK07g的克隆与表达分析

Cloning and expression analysis of cold induced protein kinase gene CIPK07g in rice（Oryza sativa L.）

摘要: CIPK 是一类Ca2+ 依赖的蛋白激酶超基因家族。对水稻中该基因家族成员之一CIPK07g 进行克隆，并对其编码蛋白的信号肽、跨膜结构、结构功能域、时空表达特性和低温处理的表达特征进行分析。结果表明，CIPK07g 基因cDNA 全长1 891 bp，包含279 bp 5′UTR 及259 bp 3′UTR，编码450 个氨基酸组成的多肽链。该基因蛋白N 端无信号肽，含跨膜结构域，为亲水蛋白，具有CIPK 基因家族特有的保守结构域，在叶片中表达较高，低温刺激后24 h 表达达到高峰。

Abstract: The gene CIPK07g in rice was cloned，which was one of the members of Ca2+-dependent protein kinase superfamily，and it’s signal peptide，transmemberane domain，conserved domain，expression profile in different organisms and low temperature treatment were analyzed. The results showed that，the cDNA full length of CIPK07g gene was 1 891 bp，including 279 bp 5′UTR and 259 bp 3′UTR，which encoded a polypeptide with 450 amino acids. There was a characteristic conserved domain of CIPK gene family in CIPK07g protein，and the protein was a hydrophilicity protein which included a transmemberane domain but had no signal peptide in N-terminal analyzed by bioinformatic tools. The CIPK07g gene was highly expressed in leaf after 24 h cold treatment.