Abstract: Peppers and tomatoes are important economical agricultural crops，several viruses cause yield losses of pepper and tomato. In 2013-2014，253 pepper and 289 tomato virus disease samples were collected in Hainan province，7 main viruses and the relative content of virus were measured by double antibody sandwich enzyme-linked immunosorbent assay（DAS-ELISA）. The results showed that the overall detection rate of pepper virus disease was 59.68% and CMV was the highest（41.11%）. There were 7 compound infection types and infection rate was 30.83%， CMV and TMV were the advantage virus sources of pepper. The overall detection rate of tomato virus disease was 44.64% and TYLCV was the highest（22.84%）. There were 10 compound infection types and infection rate was 15.57%， TYLCV and CMV were the advantage virus sources of tomato. Determination of the relative content of pepper samples showed that CMV was the highest and BBWV was the lowest. Determination of the relative content of tomato samples showed that TYLCV was the highest and BBWV was the lowest. The content of CMV was higher in pepper samples than that in tomato samples，but the content of other viruses were lower.