甜瓜 ERF 转录因子基因 CmERFII-11cDNA 克隆及表达分析

Cloning and expression analysis of ERF transcription factor gene CmERFII-11 cDNA in melon（Cucumis melo L.）

摘要: 根据已报道的甜瓜 CmERFII-11 基因 cDNA 序列设计特异性引物，采用 RT-PCR 技术从甜瓜果实中克隆得到 CmERFII-11 基因。采用相关分析软件对该基因进行了生物信息学分析，利用荧光实时定量 PCR进行表达分析。结果表明，所克隆的 cDNA 长度为 954 bp，编码 255 个氨基酸，编码蛋白质的分子量为 28.33ku，理论等电点为 7.62。该基因在根、茎、叶组织以及授粉后不同时期果实中均有表达，在叶片组织中的表达量最高。

Abstract: The specific primers were designed according to reported cDNA sequences of CmERFII-11 in melon， CmERFII-11 gene cDNA was cloned from fruit of Cucumis melo L. by RT-PCR method. CmERFII-11 gene was analyzed by the tools of bioinformatics， expression pattern was showed by Real-time quantitative PCR. The length of cDNA was 954 bp coding a polypeptide of 255 amino acids with a calculated molecular weight of 28.33 ku and an isoelectric point of 7.62. CmERFII-11 gene expressed in the root， stem， leaf and different developmental stages of fruit after pollination， CmERFII-11 gene expressed highest in leaf.