猪伪狂犬病病毒双重 PCR 鉴别方法的建立

Establishment of duplex PCR assay for identification of pseudorabies virus

摘要: 根据 GenBank 已发表的猪伪狂犬病毒（PRV）的 gB、 gE 基因序列，分别设计了 2 对特异性引物，建立了一种鉴别诊断猪伪狂犬病病毒野毒与疫苗毒的双重 PCR 检测方法。应用该方法可从野毒基因组中扩增出与预期大小相符的 2 条特异性条带，分别为 122 bp（gE）和 246 bp（gB）；从疫苗株基因组中仅扩增出 1 条特异性条带，为 246 bp（gB）。结果显示，该方法灵敏度高、特异性强，适用于 PRV 的临床诊断和流行病学调查。

Abstract: Based on the gB and gE genes of pseudorabies virus（PRV）， two pairs of special primers were designed， duplex PCR for distinguishing the wild strain and vaccine strain of PRV was established. By this duplex PCR assay， two specific fragments of 122 bp（gE）and 246 bp（gB）were simultaneously amplified from field isolate DNA， only a 246 bp（gB）fragment was amplified from vaccine strain DNA. The results indicated that the method was specific， sensitive， and could be used for rapid diagnosis and epidemiological investigation of PRV infection.