Abstract: In this study, the effects of different temperatures on the embryonic development of Oxyeleotris marmorata and morphological observation of post-embryonic development were examined. The results indicated that, in a certain temperature range, with the water temperature rising, the speed of hatching was expedited. The optimum temperature on embryonic development was 26.5-29.0益, with hatching rate of 86%, and mortality rate of newly hatched larvae less than 15%. At 29益, the effective accumulated temperature was 1 185.7 益窑h in embryonic development, and the threshold temperature was calculated as 13.8益. The post-embryonic development (mainly the larvae stage) of O. marmorata could be divided into two periods (yolk sac larva and late larva) and eight stages, including hatching larva stage, rudiment of pectoral fin stage, xanthic eye stage, melanoid eye stage, emergence of air bladder stage, exhaustion of yolk stage, chordal tip lifting stage and formation of fin stage. The newly-hatched larva was transparent, and the average length was 2.8-3.2 mm. Its nutrition was mainly raised by yolk. Once the mouth and its digestive tract were fully developed, larva was able to feed and turned into the mixed vegetation period. 120 -144 h after hatching, the larva depended on exterior nutrition completely since yolk was exhausted. Thereafter, coccygeal vertebra started to become warped, to form the caudal fin, dorsal fin, anal fin and pelvic fin, to present preliminary fish signs.