1，2，4- 三氯苯对斑马鱼肝细胞DNA 的损伤作用
Damage effects of 1，2，4-TCB on DNA of zebrafish hepatocyte measured by comet assay
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摘要: 为了研究1，2，4- 三氯苯（1，2，4-TCB）对斑马鱼（Danio retio）肝细胞DNA 的损伤作用，采用不同浓度的1，2，4-TCB 溶液（0、1、5、10 mg/L）对体外培养肝细胞进行染毒，应用单细胞凝胶电泳（彗星实验）检测肝细胞DNA 的损伤效应。结果发现，染毒2 h 后，与对照组相比，当1，2，4-TCB 浓度大于5 mg/L 时，各染毒组细胞尾长、尾部DNA 百分率均极显著升高，即1，2，4-TCB 染毒引起了肝细胞DNA 的严重断裂；随着1，2，4-TCB 浓度的增加，DNA 损伤程度加剧，细胞尾长、尾部DNA 百分率及尾距与1，2，4-TCB 染毒浓度呈明显的剂量- 效应关系。结果表明，水体中残留的1，2，4-TCB 可导致斑马鱼肝细胞DNA 损伤。Abstract: In order to explore the DNA damage in cells induced by 1，2，4-TCB，hepatocyte cells of zebrafish cultured in vitro were exposed to different concentrations of 1，2，4-TCB（0，1，5，10 mg/L）for 2 h. Then，the comet assay was applied to measure the DNA damage. Results showed that tail length，tail DNA rate of hepatocyte cells both significantly increased when treated with more than 5 mg/L 1，2，4-TCB compared with the control group. Meanwhile，the DNA damage increased gradually with the increase of 1，2，4-TCB concentration. There was an obvious dose relationship between 1，2，4-TCB concentration and DNA damage（tail length，tail DNA rate，tail moment）. It was concluded that 1， 2，4-TCB could induce DNA damage in hepatocyte cells of zebrafish
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