山莓叶抑制家兔离体肠管运动活性物的分离纯化及活性研究

Isolation，purification and activity of active componentsfrom Rubus corchorifolius leaves and their inhibitioneffects on movement of isolated rabbit intestinal

摘要: 为明确山莓叶中抗腹泻的药效成分，利用溶剂萃取、常压柱层析、中压柱层析对山莓叶中的活性成分进行分离纯化，采用家兔离体肠实验检测抗腹泻活性，并对活性单体进行结构鉴定。结果表明：山莓叶中抑制离体肠效果好的化合物是对羟基肉桂酸乙酯，当其浓度为20 mg/mL 时，离体肠张力增量的变化率达到70.0%，强于阿托品的抑制效果。

Abstract: In order to ensure the anti-diarrhea components from Rubus corchorifolius leaves，the active componentsfrom R. corchorifolius leaves were isolated and purified using solvent extraction，column chromatography and middlepressure column chromatography，and their anti-diarrhea activities were determined through the movement of isolatedintestinal of rabbit，meanwhile，the structure of active monomer was identified. The results showed that the componentwhich had anti-diarrhea activity on isolated intestinal was P-hydroxy ethyl cinnamate in R. corchorifolius leaves，whenthe concentration was 20 mg/mL，the change rate of tension increment of isolated intestinal reached 70.0%，and itsinhibition effect was better than that of atropine.