Abstract: Angelica sinensis seeds were treated with different concentrations of garlic root exudates，to study the effects of garlic root exudates on seed germination and seedling growth of A. sinensis. The results showed that the promotion effect of garlic root exudates on germination rate，germination potential and germination index increased first and then declined，and the promotion effect was significant at low concentration（0.2 mg/mL）. α-amylase activity of germinating seeds treated by 0.2 mg/mL garlic root exudates was significantly higher than that of control group. Low concentration treatment（0.1-0.2 mg/mL）promoted the accumulation of soluble sugar in A. sinensis seedlings，increased root-crown ratio and didn’t cause significant damage to seedling cells. The results indicated that garlic root exudates with low concentration（0.2 mg/mL）could effectively improve the seed germination，promote sugar accumulation in seedling leaves，improve the resistance of seedlings so as to form strong seedlings and lay the foundation for good quality and high production of A. sinensis.