Abstract: In this study, the wild Pinctada fucata from Sanya, Shenzhen and Vietnam were used as brood stock to construct 30 full-sibs families, and the polychaetes infection of 12 families with higher survival were analyzed. The selected families of F10, F11, F3 and F12 showed rapid growth, and the shell height improved by 16.01%, 14.93%, 10.51% and 10.16%, and the body weight improved by 35.72%, 28.02%, 25.98% and 17.61% compared with family F7 with the slowest growth. The families of F8, F9, F11 and F10 had the lowest polychaetes infection, and decreased by 48%, 34%, 27% and 23% compared with family F3 with the highest infestation. It demonstrated that the above 4 families improved the polydoraiosis resistance preliminarily. Those selected families could be used as the core breeding population for improving polydoraiosis resistance of P. fucata. In addition, the results also provided a reference for cultured P. fucata with selecting fast growth and polydoraiosis resistance.