Abstract: Twenty -nine strains with morphological characteristics of Bifidobacterium were isolated by plate smearing method from the feces of Guizhou Xiang pigs, aming which 6 strains had good cholesterol-reducing, acidresisting and bile salt-resisting ability, and BZ24 had the best cholesterol-reducing ability and the cholesterolremoving rate reached 33.39%. By physiological and biochemical identification, they were one strain of Bifidobacteria choerinum, one strain of B. adolescentis, two strains of B. suis, two strains of B. animalis.